Evaluation of PCR-Restriction Profile Analysis and IS 2404 Restriction Fragment Length Polymorphism and Amplified Fragment Length Polymorphism Fingerprinting for Identification and Typing of Mycobacterium ulcerans and M. marinum
نویسندگان
چکیده
منابع مشابه
Restriction fragment length polymorphism typing of Mycobacterium tuberculosis.
Restriction fragment length polymorphism (RFLP) typing of Mycobacterium tuberculosis is a recently developed technique which allows strains of tuberculosis to be differentiated with a high degree of confidence. The application of this technique has enabled the patterns of transmission of tuberculosis to be elucidated with far greater certainty than was previously possible. This review will disc...
متن کاملRestriction Fragment Length Polymorphism (RFLP)
Restriction Fragment Length Polymorphism (RFLP) is a technique in which organisms may be differentiated by analysis of patterns derived from cleavage of their DNA. If two organisms differ in the distance between sites of cleavage of a particular restriction endonuclease, the length of the fragments produced will differ when the DNA is digested with a restriction enzyme. The similarity of the pa...
متن کاملRapid Identification of Rhizobia by Restriction Fragment Length Polymorphism Analysis of PCR-Amplified 16S rRNA Genes.
Forty-eight strains representing the eight recognized Rhizobium species, two new Phaseolus bean Rhizobium genomic species, Bradyrhizobium spp., Agrobacterium spp., and unclassified rhizobia from various host plants were examined by restriction fragment length polymorphism (RFLP) analysis of 16S rRNA genes amplified by polymerase chain reaction (PCR). Twenty-one composite genotypes were obtained...
متن کاملDirect early identification of Mycobacterium tuberculosis by PCR-restriction fragment length polymorphism analysis from clinical samples.
We attempted to apply the PCR-restriction fragment length polymorphism (PCR-RFLP) technique for the early detection and identification of Mycobacterium tuberculosis directly from clinical samples. PCR-RFLP of hsp65 was applied to the DNA extracted directly from sputum samples (n = 226) collected from 226 patients. We could detect and identify M. tuberculosis in 84.5% of the acid-fast bacillus (...
متن کاملGenetic Heterogeneity among Leishmania major Isolates in Iran Determined by Restriction Fragment Length Polymorphism (RFLP) and Multilocus Microsatellite Typing (MLMT)
Background & Aims: In recent years, molecular methods for characterizing genetic heterogeneity have found a major place in modern approaches. In this study, two different molecular techniques including Restriction Fragment Length Polymorphism (RFLP) and Multi Locus microsatellite typing (MLMT) were carried out in order to evaluate genetic heterogeneity among isolates of Leishmania major in Iran...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 2001
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.39.9.3272-3278.2001